科学研究情况简介 |
【近5年来主要研究课题】 (1) 角蛋白生物材料的制备与活性评价； (2) 角蛋白在组织工程材料中的应用； (3) 角蛋白生物质资源的绿色处理与高值化利用； (4) 角蛋白酶的蛋白质工程改造与生物信息学辅助设计； (5) 角蛋白酶基因的挖掘、克隆与异源表达。 【近5年来代表性论文】 [1] Su C, Gong J S, Qin A Q, Li H, Li H, Qin J F, Qian J Y, Xu Z H, Shi J S. A combination of bioinformatics analysis and rational design strategies to enhance keratinase thermostability for efficient biodegradation of feathers[J]. Science of the Total Environment, 2022, 151824. [2] Su C, Gong J S, Qin J F, Li H, Li H, Xu Z H, Shi J S. The tale of a versatile enzyme: Molecular insights into keratinase for its industrial dissemination[J]. Biotechnology Advances, 2020, 45(3):107655. [3] Su C, Gong J S, Sun Y X, Qin J F, Zhai S, Li H, Li H, Lu Z M, Xu Z H, Shi J S. Combining Pro-peptide Engineering and Multisite Saturation Mutagenesis to Improve the Catalytic Potential of Keratinase[J]. ACS Synthetic Biology, 2019, 8 (2): 425-433. [4] Su C, Gong J S, Qin J F, He J M, Zhou Z C, Jiang M, Xu Z H, Shi J S. Glutathione enables full utilization of wool wastes for keratin production and wastewater decolorization[J]. Journal of Cleaner Production, 2020, 270: 122092. [5] Su C, Gong J S, Ye J P, He J M, Li R Y, Jiang M, Geng Y, Zhang Y, Chen J H, Xu Z H, Shi J S. Enzymatic Extraction of Bioactive and Self-Assembling Wool Keratin for Biomedical Applications[J]. Macromolecular Bioscience, 2020, 20 (9): e2000073. (Front Cover 封面) [6] Su, C, Gong, J S, Zhang, R X, Tao, L Y, Xu Z H, Shi, J S. A novel alkaline surfactant-stable keratinase with superior feather-degrading potential based on library screening strategy. International journal of biological macromolecules, 2017, 95, 404-411. [7] Ye J P, Gong J S, Su C, Liu Y G, Jiang M, Pan H P, Li R Y, Geng Y, Xu Z H, Shi, J S. Fabrication and characterization of high molecular keratin based nanofibrous membranes for wound healing[J]. Colloids and Surfaces B: Biointerfaces, 2020, 194. [8] Zhang R X, Gong J S, Su C, Qin J F, Li H, Li H, Shi J S, Xu Z H. Recombinant expression and molecular engineering of the keratinase from Brevibacillus parabrevis for dehairing performance[J]. Journal of Biotechnology, 2020, 320: 57-65. [9] Tao L Y, Gong J S, Su C, Jiang M, Li H, Li H, Lu Z M, Xu Z H, Shi J S. Mining and Expression of a Metagenome-Derived Keratinase Responsible for Biosynthesis of Silver Nanoparticles[J]. ACS Bio-materials Science and Engineering, 2018, 4: 1307-1315. 【近5年主要授权专利】 [1] Shi J S; Xu Z H; Gong J S; Li H; Su C. An improved industrial keratinase via genetic engineering and use thereof [P], 美国专利, 2018.4.9,US16/343,630 [2] 龚劲松，陶丽妍，苏畅，许正宏，史劲松. 一种经热稳定性改造的角蛋白酶突变体[P]，中国专利，CN2018109332265 |
